HT29-Luc
CBP30001L
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| I. Introduction | |
| Host Cell: | HT-29 TP53,p.R273H |
| Expressed gene: | Luciferase |
| Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+2μg/ml Puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen |
| II. Description of Host Cell Line | |
| Organism: | Homo sapiens,human |
| Tissue: | Colon |
| Disease: | Colorectal adenocarcinoma |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| III. Representative Data | |
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Figure 1. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly recommend purchasing from Cobioer).The number of Luminescence signal is 580±32(Mean±SD) on HT29 cells while 94500±5491(Mean±SD) on HT29-Luc cells. |
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| IV. Stablity Test | |
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Figure 2. Stability test results. HT29-Luc cells showed at least 32 passage stability with similar luminescence unit. |
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| V. In VIVO Data | |
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Index |
Content |
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Animal |
BALB/c-nude |
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Implanted Position |
结肠原位 |
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Injected Cell Numbers |
5×10^6/20μL |
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Matrix gel |
Without matrix gel |
1. Weight Change
Figure 3. HT29-Luc Orthotopic Tumor Model.
2. Tumor Flux
Figure 4. HT29-Luc Orthotopic Tumor Model.
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
